ANA, IFA, Cascade and Rheumatoid Arthritis Panel 2, with Reflexes
Quest Diagnostics
Sample type: Serum
Requires fasting: No
Phlebotomy required
Details
ANA, IFA, Cascade and Rheumatoid Arthritis Panel 2, with Reflexes - This test can be helpful in the diagnosis of autoimmune rheumatic diseases. Specimens with positive results for antinuclear antibodies (ANA) by indirect immunofluorescence assay (IFA) are subsequently tested for a series of ANA-specific autoantibodies in cascade tiers. This screen also includes the following markers of rheumatoid arthritis: rheumatoid factor, anti-cyclic citrullinated peptide antibody and anti-mutated citrullinated vimentin antibody.
Reflex testing uses predefined clinical logic to automatically order one or more follow-up tests based on the initial test results. Reflex tests are performed automatically without the intervention of the ordering practitioner. They are conducted using the same sample your Patient already provided. If reflex tests are required, there will be additional charges.
Initial test
- Rheumatoid factor
- Antinuclear Antibodies Screen (IFA)
- Cyclic Citrullinated Peptide (CCP) Antibody (IgG)
- Mutated Citrullinated Vimentin (MCV) Antibody
Possible reflex biomarkers
- ANA Titer
- ANA Pattern
- Chromatin (Nucleosomal) Antibody
- DNA (ds) Antibody
- RNP Antibody
- Sm Antibody
- Sm/RNP Antibody
- Jo-1 antibody
- Sjogren's antibody (ss-a)
- Sjogren's antibody (ss-b)
- Scleroderma (Scl-70) Antibody
- Ribosomal P Antibody
- Centromere b antibody
Estimated days for results: Results should be available within 2 - 5 business days after samples are received at the lab.
ANA, IFA, Cascade and Rheumatoid Arthritis Panel 2, with Reflexes - This test can be helpful in the diagnosis of autoimmune rheumatic diseases. Specimens with positive results for antinuclear antibodies (ANA) by indirect immunofluorescence assay (IFA) are subsequently tested for a series of ANA-specific autoantibodies in cascade tiers. The first tier focuses on autoantibodies associated with systemic lupus erythematosus (SLE) and mixed connective tissue disease; the second tier detects autoantibodies seen in Sjogren syndrome, systemic sclerosis and polymyositis; and the third tier includes markers of the limited form of systemic sclerosis and neuropsychiatric SLE. This screen also includes the following markers of rheumatoid arthritis: rheumatoid factor, anti-cyclic citrullinated peptide antibody, and anti-mutated citrullinated vimentin antibody.
Note that the cascade stops with the first tier in which a positive antibody result is observed. Therefore, even if an antibody included in a subsequent tier is present, it will not be reported with this test. If the ANA IFA result is positive but all the specific autoantibodies tested in the cascade antibody tests are negative, the patient may still have an autoimmune disease other than those typically associated with the antibodies tested.
- Test FAQ: Tests for Autoimmune Diseases (ANA)
- Clinical Focus: Systemic Sclerosis: Laboratory Markers for Diagnosis and Prognosis
2–5 business days
Blood Draw Options with Fullscript
Fullscript offers several convenient phlebotomy options to complete your lab testing:
Quest Diagnostics Patient Service Centers (PSC)
- Location: Visit a nearby Quest Diagnostics Patient Service Center.
- Cost: $10 sample collection fee (paid during lab order checkout).
- Scheduling: Schedule your appointment here. Appointments are recommended to reduce wait times. Walk-ins are accepted, but may experience delays. Aim to arrive 10 minutes before your appointment.
- Collection Instructions:
- Bring a valid ID.
- Bring your digital or printed requisition form, available in the Labs tab of your Fullscript account.
- Check-in: Scan the QR code from your confirmation email or follow kiosk instructions.
- More Info: Quest Diagnostics blood draw guide
Getlabs Mobile Phlebotomy
- Location: Sample collection at your home or office.
- Cost: $85 sample collection fee (paid during lab order checkout).
- Availability: Available in most locations – enter your ZIP code during checkout to confirm.
- Scheduling: Schedule your appointment here through your Fullscript account.
- Collection Instructions:
- Have your digital or printed requisition form and valid ID ready.
- Your phlebotomist will contact you by phone to coordinate arrival.
- More Info: Getlabs blood draw guide
- Preferred Specimen(s): 4 mL serum
- Alternative: NA
- Minimum Volume: 2 mL
- Collection Instructions: NA
- Transport Container: Transport tube
- Transport Temperature: Room Temperature
- Specimen Stability:
- Room temperature: 4 days
- Refrigerated: 7 days
- Frozen: 21 days
- Reject Criteria:
- Gross hemolysis • Grossly lipemic • Grossly icteric
State restrictions:
Patient can't order this test if they are located in Puerto Rico, Hawaii, Rhode Island, New Jersey, or New York
