Autoimmune Hepatitis Diagnostic Panel
Quest Diagnostics
Sample type: Serum
Requires fasting: No
Phlebotomy required
Details
Autoimmune Hepatitis Diagnostic Panel - This panel may be helpful in the diagnostic evaluation of individuals with suspected autoimmune hepatitis (AIH). This panel includes an antinuclear antibody (ANA) immunofluorescence assay (IFA) screen, performed on human epithelial type 2 (HEp-2) cells, with reflex to titer and pattern; actin (smooth muscle) antibody (SMA); liver kidney microsome type 1 (LKM-1) antibody (IgG); and anti-mitochondrial antibody (AMA) with reflex to titer.
Reflex testing uses predefined clinical logic to automatically order one or more follow-up tests based on the initial test results. Reflex tests are performed automatically without the intervention of the ordering practitioner. They are conducted using the same sample your Patient already provided. If reflex tests are required, there will be additional charges.
Initial test
- Actin (Smooth Muscle) Antibody (IgG)
- Antinuclear Antibodies Screen (IFA)
- Liver Kidney Microsomal (LKM-1) Antibody (IgG)
- Mitochondrial Antibody Screen
Possible reflex biomarkers
- ANA Titer
- ANA Pattern
- Mitochondria M2 Antibody, IFA
Estimated days for results: Results should be available within 2 - 5 business days after samples are received at the lab.
Autoimmune Hepatitis Diagnostic Panel - This panel may be helpful in the diagnostic evaluation of individuals with suspected autoimmune hepatitis (AIH). This panel includes an antinuclear antibody (ANA) immunofluorescence assay (IFA) screen, performed on human epithelial type 2 (HEp-2) cells, with reflex to titer and pattern; actin (smooth muscle) antibody (SMA); liver kidney microsome type 1 (LKM-1) antibody (IgG); and anti-mitochondrial antibody (AMA) with reflex to titer.
The diagnosis of AIH is based on clinical features, serologic profile, and exclusion of resembling diseases such as viral hepatitis [1]. The detection of antibodies associated with AIH helps establish the diagnosis and differentiate AIH types. ANA and SMA are characteristic of type 1 AIH, while LKM-1 antibody is characteristic of type 2 AIH [2]. ANAs are detected in 75% of patients with type 1 AIH but are also common in many other autoimmune diseases [1]. SMAs are present in approximately 85% of patients with type 1 AIH but can be detected in a range of other conditions, such as viral hepatitis, malignancies, rheumatic diseases, and nonalcoholic fatty liver disease [1]. LKM-1 antibody is specific for type 2 AIH and usually is not concurrent with ANA and SMA.
Up to 20% of patients with AIH are negative for ANA, SMA, and LKM-1 antibody. In these patients, testing antibodies associated with AIH, such as AMA, may help support the diagnosis [2]. AMA is the serologic hallmark of primary biliary cholangitis (PBC) but can also be detected in PBC-AIH overlap syndrome and type 1 AIH [1].
The results of this test should be interpreted in the context of pertinent clinical and family history and physical examination findings.
- Neuberger J, et al, eds. Autoimmune Liver Disease: Management and Clinical Practice. 1st ed. John Wiley & Sons Ltd, 2020.
- Mack CL, et al. Hepatology. 2020;72(2):671-722.
2–5 business days
Blood Draw Options with Fullscript
Fullscript offers several convenient phlebotomy options to complete your lab testing:
Quest Diagnostics Patient Service Centers (PSC)
- Location: Visit a nearby Quest Diagnostics Patient Service Center.
- Cost: $10 sample collection fee (paid during lab order checkout).
- Scheduling: Schedule your appointment here. Appointments are recommended to reduce wait times. Walk-ins are accepted, but may experience delays. Aim to arrive 10 minutes before your appointment.
- Collection Instructions:
- Bring a valid ID.
- Bring your digital or printed requisition form, available in the Labs tab of your Fullscript account.
- Check-in: Scan the QR code from your confirmation email or follow kiosk instructions.
- More Info: Quest Diagnostics blood draw guide
Getlabs Mobile Phlebotomy
- Location: Sample collection at your home or office.
- Cost: $85 sample collection fee (paid during lab order checkout).
- Availability: Available in most locations – enter your ZIP code during checkout to confirm.
- Scheduling: Schedule your appointment here through your Fullscript account.
- Collection Instructions:
- Have your digital or printed requisition form and valid ID ready.
- Your phlebotomist will contact you by phone to coordinate arrival.
- More Info: Getlabs blood draw guide
- Preferred Specimen(s): 2.5 mL serum
- Alternative: NA
- Minimum Volume: 0.6 mL
- Collection Instructions: NA
- Transport Container: Transport tube
- Transport Temperature: Refrigerated (Cold Packs)
- Specimen Stability:
- Room temperature: 4 days
- Refrigerated: 7 days
- Frozen: 30 days
- Reject Criteria:
- Hemolysis • Lipemia
State restrictions:
Patient can't order this test if they are located in Puerto Rico, Hawaii, Rhode Island, New Jersey, or New York
