
QuestAssureD™ 25-Hydroxyvitamin D (D2, D3)
Quest Diagnostics
Sample type: Serum
Requires fasting: No
Phlebotomy required
Details
This test measures the levels of Vitamin D in the body, which is crucial for bone health. It checks for Vitamin D activity, except in cases of renal disease.
- Vitamin D, 25-OH, Total
- 25-OH Hydroxy Vitamin D (Cholecalciferol D3)
- 25-OH Hydroxy Vitamin D (Ergocalciferol D2)
Estimated days for results: Results should be available in 2 - 5 business days after the sample is collected
Details
QuestAssureD™ 25-Hydroxyvitamin D Test - This test measures the levels of Vitamin D in the body, which is crucial for bone health. It checks for Vitamin D activity, except in cases of renal disease. Low levels might indicate a dietary deficiency, poor absorption, or liver issues, leading to bone diseases like rickets and osteomalacia. High levels could cause hypercalcemia. The test specifically measures the two common forms of Vitamin D: 25-OH D3 and 25-OH D2.
What patients is this test right for?
This test is suitable for individuals showing symptoms of Vitamin D deficiency or those at risk of bone diseases.
Symptoms of Vitamin D Deficiency:
- Bone pain and weakness
- Muscle weakness
- Mood changes, like depression
- Severe fatigue
What type of sample is required for this test?
0.5 mL serum. A simple blood draw is needed.
Test Resources https://education.questdiagnostics.com/faq/FAQ199
3–5 business days
Vitamin D, 25-OH, Total 30-100 ng/mL
Vitamin D, 25-OH, D3 Not established
Vitamin D, 25-OH, D2 Not established
Vitamin D, 25-OH, D3 Not established
Vitamin D, 25-OH, D2 Not established
Fasting for 8 hours before your blood draw is recommended but not required.
- Fasting before a blood draw means you don't eat or drink anything except water.
- Your doctor should provide any special instructions related to fasting for your test. Always follow your doctor's instructions.
Blood Draw Options with Fullscript
Fullscript offers several convenient phlebotomy options to complete your lab testing:
Quest Diagnostics Patient Service Centers (PSC)
- Location: Visit a nearby Quest Diagnostics Patient Service Center.
- Cost: $10 sample collection fee (paid during lab order checkout).
- Scheduling: Schedule your appointment here. Appointments are recommended to reduce wait times. Walk-ins are accepted, but may experience delays. Aim to arrive 10 minutes before your appointment.
- Collection Instructions:
- Bring a valid ID.
- Bring your digital or printed requisition form, available in the Labs tab of your Fullscript account.
- Check-in: Scan the QR code from your confirmation email or follow kiosk instructions.
- More Info: Quest Diagnostics blood draw guide
Getlabs Mobile Phlebotomy
- Location: Sample collection at your home or office.
- Cost: $85 sample collection fee (paid during lab order checkout).
- Availability: Available in most locations – enter your ZIP code during checkout to confirm.
- Scheduling: Schedule your appointment here through your Fullscript account.
- Collection Instructions:
- Have your digital or printed requisition form and valid ID ready.
- Your phlebotomist will contact you by phone to coordinate arrival.
- More Info: Getlabs blood draw guide
Specimen
- Preferred Specimen(s): 0.5 mL serum
- Alternative: NA
- Minimum Volume: 0.3 mL
- Collection Instructions: NA
Storage and shipping instructions
- Transport Container: Transport tube
- Transport Temperature: Room Temperature
- Specimen Stability:
- Room temperature: 21 days
- Refrigerated: 21 days
- Frozen: 21 days
- Reject Criteria: NA
State restrictions:
Patient can't order this test if they are located in Hawaii, Puerto Rico, New Jersey, New York, or Rhode Island




