
QuestAssureD™ for Infants, 25-Hydroxyvitamin D
Quest Diagnostics
Sample type: Serum
Requires fasting: No
Phlebotomy required
Details
QuestAssureD™ for Infants, 25-Hydroxyvitamin D - While measurements of 25(OH)D provide a good index of circulating Vitamin D activity in patients not suffering from renal disease, for infants less than 3 years of age it is important to remove circulating levels of the inactive c3-epimer of Vitamin D from the sample so that total Vitamin D levels are not falsely elevated. Falsely elevated results could erroneously make the child appear to be within normal limits and result in potential under-treatment. Levels of c3-epimer in older children and adults are usually negligible and do not cause falsely elevated Vitamin D results.
- Vitamin D, 25-OH, Total
- 25-OH Hydroxy Vitamin D (Cholecalciferol D3)
- 25-OH Hydroxy Vitamin D (Ergocalciferol D2)
Estimated days for results: Results should be available within 2 - 5 business days after samples are received at the lab.
QuestAssureD™ for Infants, 25-Hydroxyvitamin D - While measurements of 25(OH)D provide a good index of circulating Vitamin D activity in patients not suffering from renal disease, for infants less than 3 years of age it is important to remove circulating levels of the inactive c3-epimer of Vitamin D from the sample so that total Vitamin D levels are not falsely elevated. Falsely elevated results could erroneously make the child appear to be within normal limits and result in potential under-treatment. Levels of c3-epimer in older children and adults are usually negligible and do not cause falsely elevated Vitamin D results.
This assay employs advanced liquid chromatography - tandem mass spectrometry, which enables the chromatographic separation of the c3-epimer from 25-OH Vitamin D, thereby allowing accurate measurement in the presence of c3-epimer. While this assay will produce accurate Vitamin D results on patients of any age, it is specifically indicated for infants less than 3 years of age.
Test Resources
- Test FAQ: Vitamin D Testing
2–5 business days
Vitamin D, 25-OH, D2 Not established
Vitamin D, 25-OH, D3 Not established
Blood Draw Options with Fullscript
Fullscript offers several convenient phlebotomy options to complete your lab testing:
Quest Diagnostics Patient Service Centers (PSC)
- Location: Visit a nearby Quest Diagnostics Patient Service Center.
- Cost: $10 sample collection fee (paid during lab order checkout).
- Scheduling: Schedule your appointment here. Appointments are recommended to reduce wait times. Walk-ins are accepted, but may experience delays. Aim to arrive 10 minutes before your appointment.
- Collection Instructions:
- Bring a valid ID.
- Bring your digital or printed requisition form, available in the Labs tab of your Fullscript account.
- Check-in: Scan the QR code from your confirmation email or follow kiosk instructions.
- More Info: Quest Diagnostics blood draw guide
Getlabs Mobile Phlebotomy
- Location: Sample collection at your home or office.
- Cost: $85 sample collection fee (paid during lab order checkout).
- Availability: Available in most locations – enter your ZIP code during checkout to confirm.
- Scheduling: Schedule your appointment here through your Fullscript account.
- Collection Instructions:
- Have your digital or printed requisition form and valid ID ready.
- Your phlebotomist will contact you by phone to coordinate arrival.
- More Info: Getlabs blood draw guide
- Preferred Specimen(s): 0.5 mL serum
- Alternative: NA
- Minimum Volume: 0.2 mL
- Collection Instructions: NA
- Transport Container: Transport tube
- Transport Temperature: Room Temperature
- Specimen Stability:
- Room temperature: 7 days
- Refrigerated: 7 days
- Frozen: 28 days
- Reject Criteria:
- Gross hemolysis • Grossly lipemic • Plasma • Serum not separated from serum separator tube (SST) • Serum not separated from gel or clot within 48 hours • Grossly icteric
Maximum age:
3
State restrictions:
Patient can't order this test if they are located in Puerto Rico, Hawaii, Rhode Island, New Jersey, or New York

