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Quest Diagnostics

QuestAssureD™ for Infants, 25-Hydroxyvitamin D

Quest Diagnostics

Sample type: Serum

Requires fasting: No

Phlebotomy required


Details

QuestAssureD™ for Infants, 25-Hydroxyvitamin D - While measurements of 25(OH)D provide a good index of circulating Vitamin D activity in patients not suffering from renal disease, for infants less than 3 years of age it is important to remove circulating levels of the inactive c3-epimer of Vitamin D from the sample so that total Vitamin D levels are not falsely elevated. Falsely elevated results could erroneously make the child appear to be within normal limits and result in potential under-treatment. Levels of c3-epimer in older children and adults are usually negligible and do not cause falsely elevated Vitamin D results.


  • Vitamin D, 25-OH, Total
  • 25-OH Hydroxy Vitamin D (Cholecalciferol D3)
  • 25-OH Hydroxy Vitamin D (Ergocalciferol D2)

Estimated days for results: Results should be available within 2 - 5 business days after samples are received at the lab.

Details

QuestAssureD™ for Infants, 25-Hydroxyvitamin D - While measurements of 25(OH)D provide a good index of circulating Vitamin D activity in patients not suffering from renal disease, for infants less than 3 years of age it is important to remove circulating levels of the inactive c3-epimer of Vitamin D from the sample so that total Vitamin D levels are not falsely elevated. Falsely elevated results could erroneously make the child appear to be within normal limits and result in potential under-treatment. Levels of c3-epimer in older children and adults are usually negligible and do not cause falsely elevated Vitamin D results.

This assay employs advanced liquid chromatography - tandem mass spectrometry, which enables the chromatographic separation of the c3-epimer from 25-OH Vitamin D, thereby allowing accurate measurement in the presence of c3-epimer. While this assay will produce accurate Vitamin D results on patients of any age, it is specifically indicated for infants less than 3 years of age.


Test Resources

2–5 business days


Vitamin D, 25-OH, Total     30-100 ng/mL
Vitamin D, 25-OH, D2        Not established
Vitamin D, 25-OH, D3        Not established

  • Bone/Joint
  • Immune System
  • Nutrition

Blood Draw Options with Fullscript
Fullscript offers several convenient phlebotomy options to complete your lab testing:

Quest Diagnostics Patient Service Centers (PSC)

  • Location: Visit a nearby Quest Diagnostics Patient Service Center.
  • Cost: $10 sample collection fee (paid during lab order checkout).
  • Scheduling: Schedule your appointment here. Appointments are recommended to reduce wait times. Walk-ins are accepted, but may experience delays. Aim to arrive 10 minutes before your appointment.
  • Collection Instructions:
    • Bring a valid ID.
    • Bring your digital or printed requisition form, available in the Labs tab of your Fullscript account.
  • Check-in: Scan the QR code from your confirmation email or follow kiosk instructions.
  • More Info: Quest Diagnostics blood draw guide

Getlabs Mobile Phlebotomy

  • Location: Sample collection at your home or office.
  • Cost: $85 sample collection fee (paid during lab order checkout).
  • Availability: Available in most locations – enter your ZIP code during checkout to confirm.
  • Scheduling: Schedule your appointment here through your Fullscript account.
  • Collection Instructions:
    • Have your digital or printed requisition form and valid ID ready.
    • Your phlebotomist will contact you by phone to coordinate arrival.
  • More Info: Getlabs blood draw guide

 Specimen
  • Preferred Specimen(s): 0.5 mL serum 
  • Alternative: NA 
  • Minimum Volume: 0.2 mL 
  • Collection Instructions: NA 
 Storage and shipping instructions
  • Transport Container: Transport tube 
  • Transport Temperature: Room Temperature 
  • Specimen Stability: 
    • Room temperature: 7 days 
    • Refrigerated: 7 days 
    • Frozen: 28 days 
  • Reject Criteria: 
    • Gross hemolysis • Grossly lipemic • Plasma • Serum not separated from serum separator tube (SST) • Serum not separated from gel or clot within 48 hours • Grossly icteric 

Maximum age:

3

State restrictions:

Patient can't order this test if they are located in Puerto Rico, Hawaii, Rhode Island, New Jersey, or New York

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