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Quest Diagnostics

Shiga Toxins, EIA with Reflex to E. coli O157, Culture

Quest Diagnostics

Sample type: Stool

Requires fasting: No

Sample collection required at Quest Diagnostics location


Details

Shiga Toxins, EIA with Reflex to E. coli O157, Culture - STEC causes foodborne and waterborne diarrheal disease worldwide, which, if left undiagnosed, can progress to hemorrhagic colitis and/or hemolytic uremic syndrome (HUS). Since certain treatments and medications can increase the risk of HUS, prompt detection is necessary to prevent outbreaks and secondary transmission. STEC produces either one or both Shiga toxins (Stx1 and/or Stx2), both potent cytotoxins. By detecting the toxins, an EIA can detect STEC present in fecal samples or culture, regardless of the serotype or other virulence factors.


Reflex testing uses predefined clinical logic to automatically order one or more follow-up tests based on the initial test results. Reflex tests are performed automatically without the intervention of the ordering practitioner. They are conducted using the same sample your Patient already provided. If reflex tests are required, there will be additional charges.


Initial test
  • Escherichia coli Shiga-Like Toxin
Possible reflex biomarkers
  • Escherichia coli O157:H7

Estimated days for results: Results should be available in 2 - 5 business days after the sample is collected

Details
Shiga Toxins, EIA with Reflex to E. coli O157, Culture - STEC causes foodborne and waterborne diarrheal disease worldwide, which, if left undiagnosed, can progress to hemorrhagic colitis and/or hemolytic uremic syndrome (HUS). Since certain treatments and medications can increase the risk of HUS, prompt detection is necessary to prevent outbreaks and secondary transmission. STEC produces either one or both Shiga toxins (Stx1 and/or Stx2), both potent cytotoxins. By detecting the toxins, an EIA can detect STEC present in fecal samples or culture, regardless of the serotype or other virulence factors.

What type of sample is required for this test?
Stool collected in Cary-Blair stool culture transport medium or C&S transport medium

Test Resources
For more information, please consult your healthcare provider.


2–5 business days


  • Infectious Disease
  • Gastrointestinal

Collection Options with Fullscript
Sample collection requires a test kit that can be picked up at a nearby Quest Diagnostics Patient Service Center.

Quest Diagnostics Patient Service Centers (PSC)

  • Location: Visit a nearby Quest Diagnostics Patient Service Center.
  • Cost: $10 sample collection fee (paid during lab order checkout).
  • Scheduling: Schedule your appointment here. Appointments are recommended to reduce wait times. Walk-ins are accepted, but may experience delays. Aim to arrive 10 minutes before your appointment.
  • Collection Instructions:
    • Bring a valid ID.
    • Bring your digital or printed requisition form, available in the Labs tab of your Fullscript account.
  • Check-in: Scan the QR code from your confirmation email or follow kiosk instructions.
  • More Info: Quest Diagnostics collection guide

 Specimen
  • Preferred Specimen(s): Stool collected in Cary-Blair stool culture transport medium or C&S transport medium 
  • Alternative: NA 
  • Minimum Volume: 1 mL 
  • Collection Instructions: NA 
 Storage and shipping instructions
  • Transport Container: Cary-Blair stool culture transport medium 
  • Transport Temperature: Room Temperature 
  • Specimen Stability
    • Room temperature: 4 days 
    • Refrigerated: 14 days 
    • Frozen: 14 days 
  • Reject Criteria
    • Specimens in 10% formalin or other fixatives 

State restrictions:

Patient can't order this test if they are located in Hawaii, Puerto Rico, New Jersey, New York, or Rhode Island

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