
Syphilis Antibody Cascading Reflex (Reverse Algorithm)
Quest Diagnostics
Sample type: Serum
Requires fasting: No
Phlebotomy required
Details
Syphilis Antibody Cascading Reflex (Reverse Algorithm) - This test is used to aid in the diagnosis of syphilis using the reverse algorithm. The algorithm starts with the Treponema pallidum Antibody immunoassay, a treponemal serologic test. If positive, samples will be further tested using RPR, a nontreponemal (lipoidal antigen) serologic test, and if reactive will reflex to titer. T. Pallidum positive samples that do not confirm with RPR will be tested by a second treponemal test, Treponema Pallidum Particle Aggluctination (TP-PA).
Reflex testing uses predefined clinical logic to automatically order one or more follow-up tests based on the initial test results. Reflex tests are performed automatically without the intervention of the ordering practitioner. They are conducted using the same sample your Patient already provided. If reflex tests are required, there will be additional charges.
Initial test
- Treponema Pallidum Ab
Possible reflex biomarkers
- RPR (Reagin Ab)
- RPR Titer
- Treponema pallidum Ab, Agglutination
Estimated days for results: Results should be available within 2 - 5 business days after samples are received at the lab.
2–5 business days
Blood Draw Options with Fullscript
Fullscript offers several convenient phlebotomy options to complete your lab testing:
Quest Diagnostics Patient Service Centers (PSC)
- Location: Visit a nearby Quest Diagnostics Patient Service Center.
- Cost: $10 sample collection fee (paid during lab order checkout).
- Scheduling: Schedule your appointment here. Appointments are recommended to reduce wait times. Walk-ins are accepted, but may experience delays. Aim to arrive 10 minutes before your appointment.
- Collection Instructions:
- Bring a valid ID.
- Bring your digital or printed requisition form, available in the Labs tab of your Fullscript account.
- Check-in: Scan the QR code from your confirmation email or follow kiosk instructions.
- More Info: Quest Diagnostics blood draw guide
Getlabs Mobile Phlebotomy
- Location: Sample collection at your home or office.
- Cost: $85 sample collection fee (paid during lab order checkout).
- Availability: Available in most locations – enter your ZIP code during checkout to confirm.
- Scheduling: Schedule your appointment here through your Fullscript account.
- Collection Instructions:
- Have your digital or printed requisition form and valid ID ready.
- Your phlebotomist will contact you by phone to coordinate arrival.
- More Info: Getlabs blood draw guide
- Preferred Specimen(s): 3 mL serum
- Alternative: NA
- Minimum Volume: 1.4 mL
- Collection Instructions: NA
- Transport Container: Transport tube
- Transport Temperature: Room Temperature
- Specimen Stability:
- Room temperature: 4 days
- Refrigerated: 7 days
- Frozen: 30 days
- Reject Criteria:
- Hemolysis • Lipemia
State restrictions:
Patient can't order this test if they are located in Puerto Rico, Hawaii, Rhode Island, New Jersey, or New York

